10854   Nhedc2     Status: ES cell colonies screened / QC positives

GeneNhedc2
Chromosome3
Deletion Start135,342,305
Deletion End135,317,107
Deletion Size25,199
Genome Build38
MGI IDMGI:2140077
NCBI Gene ID97086
BAC ID347N20
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 3

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,317,350135,317,372CACTCGGAAGAGGGATACGGAAG
TUP135,317,374135,317,395ATTGTGAGCATGCAGCCAGCCC
TUR135,317,398135,317,416CAGGGTCTCGCCAGGTTAC
TDF135,341,351135,341,370CCAATGTCCTGCTGGCTCTG
TDP135,341,372135,341,395TCCAGTGGCCGGAAACTGTCATGT
TDR135,341,407135,341,426CTGCTGAGTCCTGCATAGTC

PCR Primers

PrimerSequence
SUTCAAGGAATGATGGGAAAGG
SDGTCCCTCCTACTCCCTTCCC
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR67 bp++-
TDFTDR76 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10854A-E10VGB6C57BL/6NTac
10854A-B6VGB6C57BL/6NTac
10854A-A1VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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