| Gene | Endod1 |
| Chromosome | 9 |
| Deletion Start | 14,380,967 |
| Deletion End | 14,356,673 |
| Deletion Size | 24,314 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 9
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 14,380,069 | 14,380,086 | TGGCGGCAGGGATCAAAC |
| TUP | 14,380,045 | 14,380,068 | ACCGAGAAAGCCTTCCATGGGCCA |
| TUR | 14,380,006 | 14,380,025 | GGAGACCTGGCAGAGGAGAG |
| TDF | 14,356,914 | 14,356,933 | TGTCTGCTGAAGGCTGTCTG |
| TDP | 14,356,890 | 14,356,913 | CCGAGCTCTGAGCATCCCTCTCCG |
| TDR | 14,356,858 | 14,356,879 | ACACAGGGAAAGTGGCTACATC |
PCR Primers
| Primer | Sequence |
| SU | CCCCTTGGGCTAAGCCAGT |
| SD | CCTAAAGTTAGCAGACGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 351 bp | - | + | + |
| TUF | TUR | 81 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 331 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10855A-C10 | VGB6 | C57BL/6NTac | |
| 10855A-D2 | VGB6 | C57BL/6NTac | |
| 10855A-F4 | VGB6 | C57BL/6NTac | |
