| Gene | Exoc8 |
| Chromosome | 8 |
| Deletion Start | 124,897,623 |
| Deletion End | 124,895,455 |
| Deletion Size | 2,169 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 124,896,865 | 124,896,884 | CCGAGAGCCGAATCTTCCAG |
| TUP | 124,896,834 | 124,896,859 | AAATGCCAAGATCAAGCGCGAGTGGC |
| TUR | 124,896,810 | 124,896,831 | TCTTGGTTTCCTCCAGCACTTC |
| TDF | 124,895,832 | 124,895,851 | TGAGCAGTACACGGGAGATG |
| TDP | 124,895,805 | 124,895,830 | CTGCTGGGTGAACTTGAGCTACACGG |
| TDR | 124,895,766 | 124,895,785 | CCTCCAGGAAGCCCATAGTC |
PCR Primers
| Primer | Sequence |
| SU | CTCCCGGCCCTGCTGTCAG |
| SD | GGTGTGACCATACACTCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 578 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10858A-G11 | VGB6 | C57BL/6NTac | |
| 10858A-B12 | VGB6 | C57BL/6NTac | |
| 10858A-C11 | VGB6 | C57BL/6NTac | |
