| Gene | Nsun7 |
| Chromosome | 5 |
| Deletion Start | 66,297,927 |
| Deletion End | 66,260,932 |
| Deletion Size | 36,996 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 66,261,541 | 66,261,562 | GGAGCCGACATGGTTGTATAGG |
| TUP | 66,261,567 | 66,261,588 | TACTCCCAGCGGCTGAGCCTTG |
| TUR | 66,261,597 | 66,261,616 | CCCATCCTGGCTTGGAACTC |
| TDF | 66,297,729 | 66,297,751 | CCTCTAGTGGCATGTGTTTCCTG |
| TDP | 66,297,753 | 66,297,775 | CACGTGACCCTTGGGCGTCTCCT |
| TDR | 66,297,790 | 66,297,814 | GGGCAAAGAGAAGACGAATTTAGTG |
PCR Primers
| Primer | Sequence |
| SU | AGTATGGGATGTTCCGATGG |
| SD | TCGCTGTGTTGTGTTGTCTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10861A-C8 | VGB6 | C57BL/6NTac | |
| 10861A-E9 | VGB6 | C57BL/6NTac | |
| 10861A-E1 | VGB6 | C57BL/6NTac | |
