| Gene | Nos1 |
| Chromosome | 5 |
| Deletion Start | 117,896,830 |
| Deletion End | 117,867,220 |
| Deletion Size | 29,611 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 117,867,239 | 117,867,258 | TCCAGCAGATCCAACCCAAC |
| TUP | 117,867,260 | 117,867,286 | TCATTTCTGTCCGTCTCTTCAAACGCA |
| TUR | 117,867,296 | 117,867,315 | CTTCACCAGGAAGCCCAGAC |
| TDF | 117,896,688 | 117,896,706 | CCACATGGACAGGCTGGAG |
| TDP | 117,896,708 | 117,896,734 | AGGTGAACAAGGAGATCGAGAGCACCA |
| TDR | 117,896,739 | 117,896,758 | CCGTGTCCTTGAGCTGGTAG |
PCR Primers
| Primer | Sequence |
| SU | CCTCTGCATCTGACAAGCTG |
| SD | AACAGAAAGCCTGATCCTAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10864A-E7 | VGB6 | C57BL/6NTac | |
| 10864C-A4 | VGB6 | C57BL/6NTac | |
| 10864C-B12 | VGB6 | C57BL/6NTac | |
