Gene | Nos1 |
Chromosome | 5 |
Deletion Start | 117,896,830 |
Deletion End | 117,867,220 |
Deletion Size | 29,611 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 117,867,239 | 117,867,258 | TCCAGCAGATCCAACCCAAC |
TUP | 117,867,260 | 117,867,286 | TCATTTCTGTCCGTCTCTTCAAACGCA |
TUR | 117,867,296 | 117,867,315 | CTTCACCAGGAAGCCCAGAC |
TDF | 117,896,688 | 117,896,706 | CCACATGGACAGGCTGGAG |
TDP | 117,896,708 | 117,896,734 | AGGTGAACAAGGAGATCGAGAGCACCA |
TDR | 117,896,739 | 117,896,758 | CCGTGTCCTTGAGCTGGTAG |
PCR Primers
Primer | Sequence |
SU | CCTCTGCATCTGACAAGCTG |
SD | AACAGAAAGCCTGATCCTAC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | bp | - | + | + |
TUF | TUR | 77 bp | + | + | - |
TDF | TDR | 71 bp | + | + | - |
NeoFwd | SD | bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
10864A-E7 | VGB6 | C57BL/6NTac | |
10864C-A4 | VGB6 | C57BL/6NTac | |
10864C-B12 | VGB6 | C57BL/6NTac | |