Information regarding availability, timelines and delivery of materials can be found at www.komp.org or www.knockoutmouse.org.

10869 Hdhd1a Status: ES cell colonies screened / QC positives

Gene	Hdhd1a
Chromosome	18
Deletion Start	50,568,657
Deletion End	50,567,936
Deletion Size	722
Genome Build	38

MGI ID	MGI:1914615
NCBI Gene ID	67365
BAC ID	171J18
BAC Library	bMQ
Cassette	ZEN-Ub1
Clones Available	Yes

Sequence Downloads and Strategy Information

Primer Genomic Coordinates on Chromosome 18

Please be aware that these oligonucleotides have been validated only by Taqman assay.

Primer	Start	End	Sequence
TUF	50,568,499	50,568,519	TGGGATGTTAAGTCCCTGGTC
TUP	50,568,465	50,568,492	AAGAAGGCTCTAGAAACCGCACAGACCA
TUR	50,568,435	50,568,459	TGGATATGGGTAAATTGAGGAACTC
TDF	50,568,033	50,568,052	CCTGAGCGCAGACCTAACAC
TDP	50,568,005	50,568,030	AAAGCCACCCTGGTGCTGAGTTCCCT
TDR	50,567,961	50,567,983	CCGTAAAGGCAGGTAGACCAAAC

PCR Primers

Primer	Sequence
SU	TAGAAGGATTCATTTCTACC
SD	ACACAGTGGTCATATTCACG
LacInR	TTGACTGTAGCGGCTGATGTTG
LacInf	GGTAAACTGGCTCGGATTAGGG
NeoInR	TACTTTCTCGGCAGGAGCAAGGTG
NeoInF	TTCGGCTATGACTGGGCACAACAG
LacInZRev	GTCTGTCCTAGCTTCCTCACTG
NeoFwd	TCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward Primer	Reverse Primer	Length	WT	HET	KO
SU	LacZRev	413 bp	-	+	+
TUF	TUR	85 bp	+	+	-
TDF	TDR	92 bp	+	+	-
NeoFwd	SD	392 bp	-	+	+
LacInf	LacInR	210 bp	-	+	+
NeoInf	NeoInR	282 bp	-	+	+

Available Clones

ES Cell ordering information can be found at the KOMP Repository.

Clone Name	Parental ES Cell	Mouse Strain	Cassette Copy Number
10869A-A2	VGB6	C57BL/6NTac
10869A-F3	VGB6	C57BL/6NTac
10869A-C11	VGB6	C57BL/6NTac