| Gene | Hebp2 |
| Chromosome | 10 |
| Deletion Start | 18,545,792 |
| Deletion End | 18,541,114 |
| Deletion Size | 4,679 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 18,545,175 | 18,545,193 | GCACTACGGACCGGCTAAG |
| TUP | 18,545,149 | 18,545,170 | TCAGCACTTGCGTGGAGTCCCT |
| TUR | 18,545,119 | 18,545,141 | AAACCAGTCTGGATGGCTGAATC |
| TDF | 18,541,984 | 18,542,008 | GGTAGTTGGGAAGCTGAGATAGAAG |
| TDP | 18,541,947 | 18,541,975 | AGCCTGAGAGTTTAGACACAGCCTAAGCA |
| TDR | 18,541,886 | 18,541,907 | CTGTGCTGTGTTGCATTTGTTC |
PCR Primers
| Primer | Sequence |
| SU | CTAGATGACCCAGGGCCAGC |
| SD | GTGCAGAATCCATCATTACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 378 bp | - | + | + |
| TUF | TUR | 75 bp | + | + | - |
| TDF | TDR | 123 bp | + | + | - |
| NeoFwd | SD | 427 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10870A-D1 | VGB6 | C57BL/6NTac | |
| 10870A-H3 | VGB6 | C57BL/6NTac | |
