| Gene | Cml2 |
| Chromosome | 6 |
| Deletion Start | 85,901,536 |
| Deletion End | 85,867,606 |
| Deletion Size | 33,931 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 85,868,332 | 85,868,354 | TACCAGGAGAAGGACCACAAAAG |
| TUP | 85,868,305 | 85,868,329 | TCCTGGAATTGTTCTCCAGCGGCAT |
| TUR | 85,868,257 | 85,868,276 | TGAGGCAGTGTCAGCATCTG |
| TDF | 85,867,903 | 85,867,922 | GCAAAGGCACTGGTCAGAAC |
| TDP | 85,867,880 | 85,867,902 | TGTCCTCCAGTTTGCTCGGGACC |
| TDR | 85,867,847 | 85,867,868 | TGCCAGTCTCAAGGACAACATC |
PCR Primers
| Primer | Sequence |
| SU | ATTAGTGAGGATGGCTTGAC |
| SD | AAGCCAGAAGCACACAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 33102 bp | - | + | + |
| TUF | TUR | 98 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 360 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10871A-F3 | VGB6 | C57BL/6NTac | |
| 10871A-D2 | VGB6 | C57BL/6NTac | |
| 10871A-A7 | VGB6 | C57BL/6NTac | |
