| Gene | Tmem141 |
| Chromosome | 2 |
| Deletion Start | 25,621,632 |
| Deletion End | 25,620,498 |
| Deletion Size | 1,135 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 25,621,211 | 25,621,230 | CTTGTGCCAGGTCCCCTTCT |
| TUP | 25,621,175 | 25,621,199 | CCACCCTCCTGCTTCCTGTTCTCCA |
| TUR | 25,621,155 | 25,621,173 | CTGGCCACAGATCCTGCAA |
| TDF | 25,621,037 | 25,621,056 | GCCCAGAGGGACAGCTAGCA |
| TDP | 25,621,008 | 25,621,033 | TCAGGAAGGCAAGCCCACAAGACTCA |
| TDR | 25,620,986 | 25,621,006 | CGAAAGGCTTCAGTCCGTCTT |
PCR Primers
| Primer | Sequence |
| SU | GAGAGCTGACCCTACGATGC |
| SD | CTGGCAGGTCCTGGATTTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 390 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 296 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10874A-A6 | VGB6 | C57BL/6NTac | |
| 10874A-B7 | VGB6 | C57BL/6NTac | |
| 10874A-D3 | VGB6 | C57BL/6NTac | |
| 10874A-F9 | VGB6 | C57BL/6NTac | |
