| Gene | Rftn1 |
| Chromosome | 17 |
| Deletion Start | 50,169,144 |
| Deletion End | 50,168,993 |
| Deletion Size | 152 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 50,169,111 | 50,169,133 | GAACAAGCTGGAGAAACGTGAAG |
| TUP | 50,169,078 | 50,169,107 | AGCGGCCTGGGAACATTTATTCAACTCTGA |
| TUR | 50,169,058 | 50,169,076 | GGTCTCCACCTGAGGTCTC |
| TDF | 50,169,083 | 50,169,105 | CGGCCTGGGAACATTTATTCAAC |
| TDP | 50,169,057 | 50,169,077 | TCTGAGAGACCTCAGGTGGAGACCA |
| TDR | 50,169,011 | 50,169,033 | AGTGTGGTGAACTCTAGAAAGCA |
PCR Primers
| Primer | Sequence |
| SU | GTGTCTTGTAATGCGTACTC |
| SD | CCTGAATGCTCAGGTAACTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 333 bp | - | + | + |
| TUF | TUR | 76 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 263 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10875A-A6 | VGB6 | C57BL/6NTac | |
| 10875A-A8 | VGB6 | C57BL/6NTac | |
| 10875A-E1 | VGB6 | C57BL/6NTac | |
