| Gene | Pmp2 |
| Chromosome | 3 |
| Deletion Start | 10,183,816 |
| Deletion End | 10,180,726 |
| Deletion Size | 3,091 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,183,209 | 10,183,229 | TCAAACTCCGTCCCACCAATG |
| TUP | 10,183,183 | 10,183,206 | TGCCCATCCTAGTGTTGAGCAGGG |
| TUR | 10,183,148 | 10,183,169 | CAGAACAAAGTCTCCACCACAC |
| TDF | 10,181,144 | 10,181,162 | CAATTACCCTGGCGGAGAC |
| TDP | 10,181,115 | 10,181,140 | CCCTCACAGACATGCCGAGAGGTTTG |
| TDR | 10,181,071 | 10,181,095 | TGTCAGCTTTGTGGGAGTTAGAATC |
PCR Primers
| Primer | Sequence |
| SU | TATAGAAGGGCTTAAGCATG |
| SD | CTAGGCTGTCACTTTGCACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 484 bp | - | + | + |
| TUF | TUR | 82 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 342 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10878A-E1 | VGB6 | C57BL/6NTac | |
| 10878A-E2 | VGB6 | C57BL/6NTac | |
| 10878B-D5 | VGB6 | C57BL/6NTac | |
| 10878B-D12 | VGB6 | C57BL/6NTac | |
| 10878B-G8 | VGB6 | C57BL/6NTac | |
