| Gene | Usp51 |
| Chromosome | X |
| Deletion Start | 153,008,516 |
| Deletion End | 153,009,483 |
| Deletion Size | 968 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,008,911 | 153,008,934 | TACAGATTGCCTGAAGTGGTTTAC |
| TUP | 153,008,935 | 153,008,957 | AAGGCCCGAAGACCTGGGTAGCA |
| TUR | 153,008,978 | 153,008,997 | TCCTGGTAGCTTTGGCATTG |
| TDF | 153,009,061 | 153,009,084 | TGAACATTTAGGCAAACAGAGACG |
| TDP | 153,009,099 | 153,009,128 | TTATCTCGTTTCCTTTGGAGCTGGACATGA |
| TDR | 153,009,152 | 153,009,173 | GCTGGCCTTTCATTATGCTTTC |
PCR Primers
| Primer | Sequence |
| SU | GATGGCATCTGAGGTGGAAG |
| SD | TATGTGACCTTTTCAACCTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 397 bp | - | + | + |
| TUF | TUR | 87 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | 352 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10888A-B4 | VGB6 | C57BL/6NTac | |
| 10888B-G12 | VGB6 | C57BL/6NTac | |
