10892   Tex24     Status: ES cell colonies screened / QC positives

GeneTex24
Chromosome8
Deletion Start27,344,449
Deletion End27,345,919
Deletion Size1,471
Genome Build38
MGI IDMGI:1921539
NCBI Gene ID541463
BAC ID368L12
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome 8

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF27,344,76927,344,791TAAGGGCGAATCTGTGATCAAAG
TUP27,344,79427,344,819CCCGAGGACAGGCAGACATTTGTTGG
TUR27,344,87727,344,898AAGCAACTCCATTGTCCTCTTC
TDF27,345,61527,345,638CAGTAGTCAGGCATGTCAGTATGG
TDP27,345,63927,345,660CACCATCTGCCCGTGGACACCA
TDR27,345,73527,345,758CCCAGAAGCTGAATGGTCTCTATG

PCR Primers

PrimerSequence
SUTGTGAAAGTCATATAGATAG
SDTGCAAGGGTACAAGATATGG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR130 bp++-
TDFTDR144 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10892A-B10VGB6C57BL/6NTac
10892A-H10VGB6C57BL/6NTac
10892A-D11VGB6C57BL/6NTac
10892A-E12VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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