| Gene | Prdx6b |
| Chromosome | 2 |
| Deletion Start | 80,292,852 |
| Deletion End | 80,293,523 |
| Deletion Size | 672 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 80,293,074 | 80,293,096 | GTTGAGGATCATCTTGCCTGGAG |
| TUP | 80,293,103 | 80,293,131 | CATCAATGCTTACAATGGTGCAACACCCA |
| TUR | 80,293,166 | 80,293,185 | GGATGGAAATGTCCCTGTCC |
| TDF | 80,293,409 | 80,293,427 | GATGGTCCTTCCCGACCTC |
| TDP | 80,293,430 | 80,293,456 | CGAAGAGGAAGCCAAACGATGTTTCCC |
| TDR | 80,293,462 | 80,293,483 | GGGAGCTTTGTGGTGGAGATTC |
PCR Primers
| Primer | Sequence |
| SU | GGTGCAAGTTTGCAACGTGC |
| SD | CTAAGGAATTGTCAGTATAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 512 bp | - | + | + |
| TUF | TUR | 112 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 389 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10898B-D11 | VGB6 | C57BL/6NTac | |
| 10898B-F4 | VGB6 | C57BL/6NTac | |
| 10898B-G9 | VGB6 | C57BL/6NTac | |
| 10898C-E10 | VGB6 | C57BL/6NTac | |
| 10898B-F5 | VGB6 | C57BL/6NTac | |
| 10898C-H1 | VGB6 | C57BL/6NTac | |
