| Gene | Cxcl3 |
| Chromosome | 5 |
| Deletion Start | 90,786,174 |
| Deletion End | 90,787,519 |
| Deletion Size | 1,346 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 90,786,442 | 90,786,461 | CCACTGCACCCAGACAGAAG |
| TUP | 90,786,462 | 90,786,487 | TCATGTGAGTGTCTCCCTGGATAGCC |
| TUR | 90,786,568 | 90,786,588 | TGGCTCTGCACAGAGAAGTAG |
| TDF | 90,787,269 | 90,787,291 | CCCTGTTCATCTCTGTCCTGTAG |
| TDP | 90,787,293 | 90,787,316 | AGAGCATCCGCAAGCCTGAGGTGT |
| TDR | 90,787,328 | 90,787,345 | TGGCCTGTGATGCCTGTG |
PCR Primers
| Primer | Sequence |
| SU | AACCTCTTCCCTCTTGCTCC |
| SD | TTTCATGTGACACCGTAAGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 147 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10903A-H4 | VGB6 | C57BL/6NTac | |
| 10903A-G8 | VGB6 | C57BL/6NTac | |
| 10903A-G4 | VGB6 | C57BL/6NTac | |
| 10903A-E9 | VGB6 | C57BL/6NTac | |
