| Gene | Zcchc18 |
| Chromosome | X |
| Deletion Start | 136,994,623 |
| Deletion End | 136,995,819 |
| Deletion Size | 1,197 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,994,889 | 136,994,909 | GCGCCTGATGAGAACCCTTAG |
| TUP | 136,994,918 | 136,994,940 | CCCGGGAGGCCATGCGTTTGTTC |
| TUR | 136,994,949 | 136,994,972 | TCTGCGACATTTAGATTGGGATTG |
| TDF | 136,995,663 | 136,995,683 | CAAGTACCCAATCCGCTGTCC |
| TDP | 136,995,693 | 136,995,714 | TGAAGAGGGCCACGCCAAAGAA |
| TDR | 136,995,719 | 136,995,741 | GCCCTTGTTGCTGGTATTGTCAC |
PCR Primers
| Primer | Sequence |
| SU | CAAAGGCCCCACTAGCACAC |
| SD | CAAAAAGGTACCACTTCACG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 420 bp | - | + | + |
| TUF | TUR | 84 bp | + | + | - |
| TDF | TDR | 79 bp | + | + | - |
| NeoFwd | SD | 348 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10904A-A9 | VGB6 | C57BL/6NTac | |
| 10904A-C2 | VGB6 | C57BL/6NTac | |
| 10904A-D6 | VGB6 | C57BL/6NTac | |
| 10904A-E12 | VGB6 | C57BL/6NTac | |
| 10904A-H7 | VGB6 | C57BL/6NTac | |
