| Gene | Ascl3 |
| Chromosome | 7 |
| Deletion Start | 109,728,107 |
| Deletion End | 109,727,567 |
| Deletion Size | 541 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 109,727,960 | 109,727,980 | TACCGGCCTCTTACACAGATG |
| TUP | 109,727,937 | 109,727,959 | AGCTGCCTCTGCTGCCCTTCTCC |
| TUR | 109,727,852 | 109,727,871 | AGTCACAGCGCCTGTAGTTG |
| TDF | 109,727,720 | 109,727,739 | GGAGAAACGGCTCAGCAAAG |
| TDP | 109,727,699 | 109,727,719 | TGGAGACCCTCAGAGCTGCCA |
| TDR | 109,727,648 | 109,727,670 | TCTCAGATTCATCCGGGTACAAG |
PCR Primers
| Primer | Sequence |
| SU | CCAGTGCCTCTACACAAATG |
| SD | GGAGAGAGCGTTCAGCCAAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 276 bp | - | + | + |
| TUF | TUR | 129 bp | + | + | - |
| TDF | TDR | 92 bp | + | + | - |
| NeoFwd | SD | 350 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10905A-A8 | VGB6 | C57BL/6NTac | |
| 10905A-A9 | VGB6 | C57BL/6NTac | |
| 10905A-A10 | VGB6 | C57BL/6NTac | |
| 10905A-B11 | VGB6 | C57BL/6NTac | |
| 10905A-D6 | VGB6 | C57BL/6NTac | |
| 10905A-E8 | VGB6 | C57BL/6NTac | |
