| Gene | Pnma3 |
| Chromosome | X |
| Deletion Start | 73,064,806 |
| Deletion End | 73,066,222 |
| Deletion Size | 1,417 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 73,064,913 | 73,064,934 | GTGAGGATGAGTTTGAGGAGAC |
| TUP | 73,064,935 | 73,064,960 | TCTGCACGAAGCTCTCAAGCATCTGG |
| TUR | 73,064,964 | 73,064,984 | TCCTGCCGATGATCCTGTATC |
| TDF | 73,066,000 | 73,066,019 | CCAGCACAGAAAGGGAGGTG |
| TDP | 73,066,020 | 73,066,043 | TGCCCAGGGATGATTCTCAAGGCA |
| TDR | 73,066,077 | 73,066,094 | GGCCATCTTCCCCACAAC |
PCR Primers
| Primer | Sequence |
| SU | CTGCAGCCTTTAGGGTAAAC |
| SD | ACATCTGTAGCAGTTCCTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 387 bp | - | + | + |
| TUF | TUR | 72 bp | + | + | - |
| TDF | TDR | 95 bp | + | + | - |
| NeoFwd | SD | 353 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10907A-A7 | VGB6 | C57BL/6NTac | |
| 10907A-A8 | VGB6 | C57BL/6NTac | |
| 10907A-E2 | VGB6 | C57BL/6NTac | |
| 10907A-E12 | VGB6 | C57BL/6NTac | |
| 10907A-F12 | VGB6 | C57BL/6NTac | |
