| Gene | Glycam1 |
| Chromosome | 15 |
| Deletion Start | 103,565,028 |
| Deletion End | 103,562,873 |
| Deletion Size | 2,156 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,564,404 | 103,564,423 | TGCACACCTGCATTTCCAAC |
| TUP | 103,564,373 | 103,564,401 | TCAGGAAGCAGAAGCAGAACTACTAAGCA |
| TUR | 103,564,320 | 103,564,342 | AGCTTCCTCAACCTCCCTTACTC |
| TDF | 103,563,251 | 103,563,271 | AGAGACCACAAGACCCACCAC |
| TDP | 103,563,225 | 103,563,250 | CTCAGCTGGTATGAGCCAGGGAAGAA |
| TDR | 103,563,201 | 103,563,220 | TTACCACCTCCCAAGACATC |
PCR Primers
| Primer | Sequence |
| SU | ACACCATGGCAGTAGCTAGG |
| SD | ACTGGGCTTGTAAACTGAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 104 bp | + | + | - |
| TDF | TDR | 71 bp | + | + | - |
| NeoFwd | SD | 354 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10916A-F3 | VGB6 | C57BL/6NTac | |
| 10916A-G2 | VGB6 | C57BL/6NTac | |
