| Gene | March4 |
| Chromosome | 1 |
| Deletion Start | 72,535,134 |
| Deletion End | 72,534,632 |
| Deletion Size | 503 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,535,036 | 72,535,053 | CAGATGTTGCGCCACCAG |
| TUP | 72,535,011 | 72,535,033 | TCTCCTCAAGTGCCGCTGTCGAA |
| TUR | 72,534,974 | 72,534,996 | CGCCTCAGTAGGAAAACCTTCAG |
| TDF | 72,534,798 | 72,534,818 | CACCACCTTTGCCTCCTTCTG |
| TDP | 72,534,771 | 72,534,794 | ATGATGACTGGGATGGCCCAGCCA |
| TDR | 72,534,699 | 72,534,722 | AGCAGTCCTCAGTTTTCTCCTTAC |
PCR Primers
| Primer | Sequence |
| SU | TGAGTTCCCTAAGCTGCTCC |
| SD | TGAGATGCTGGTAGAAGTTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 80 bp | + | + | - |
| TDF | TDR | 120 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10919A-A12 | VGB6 | C57BL/6NTac | |
| 10919A-F4 | VGB6 | C57BL/6NTac | |
| 10919A-E7 | VGB6 | C57BL/6NTac | |
| 10919A-E5 | VGB6 | C57BL/6NTac | |
