| Gene | Kbtbd7 |
| Chromosome | 14 |
| Deletion Start | 79,452,039 |
| Deletion End | 79,428,832 |
| Deletion Size | 23,208 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 14
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 79,427,200 | 79,427,218 | CGAAGCCTGTGCCTCCTTC |
| TUP | 79,452,545 | 79,452,564 | CTGGCTCGCCGCCTTGACCT |
| TUR | 79,452,601 | 79,452,620 | GCAGCTTGTGGTGGTCAAAG |
| TDF | 79,428,367 | 79,428,388 | CTACAACCCTGCTAGAGGAGAG |
| TDP | 79,428,389 | 79,428,417 | TGGAGGCGGATTAGTAATATCCCACTGGA |
| TDR | 79,428,418 | 79,428,440 | TCTGGTAGTTGTGGGTTTCTGAG |
PCR Primers
| Primer | Sequence |
| SU | CCAAGATGCAAAGCGGCTTC |
| SD | ATCATGGACTTTTAGCACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 25836 bp | - | + | + |
| TUF | TUR | 25421 bp | + | + | - |
| TDF | TDR | 74 bp | + | + | - |
| NeoFwd | SD | 268 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10920A-C2 | VGB6 | C57BL/6NTac | |
| 10920A-D4 | VGB6 | C57BL/6NTac | |
| 10920A-E3 | VGB6 | C57BL/6NTac | |
| 10920A-F7 | VGB6 | C57BL/6NTac | |
| 10920A-G12 | VGB6 | C57BL/6NTac | |
| 10920A-H7 | VGB6 | C57BL/6NTac | |
