| Gene | Defb12 |
| Chromosome | 8 |
| Deletion Start | 19,114,817 |
| Deletion End | 19,112,652 |
| Deletion Size | 2,166 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 8
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 19,114,520 | 19,114,542 | AGTACACTGGTCTTTCTGGCTTC |
| TUP | 19,114,494 | 19,114,519 | CAGGAGTGCTGGTCTTGAGAAACCCA |
| TUR | 19,114,450 | 19,114,473 | ATGGCTTTCGAGGGACTACTGATG |
| TDF | 19,113,049 | 19,113,067 | ACTCGGCTAGGCTGACCAC |
| TDP | 19,113,025 | 19,113,048 | TGAGAGTCAGCTGTCCCTGCTTCC |
| TDR | 19,112,999 | 19,113,018 | TTGTGTCACTCACGGTCCTC |
PCR Primers
| Primer | Sequence |
| SU | CAGTAGAAGTGGGCACAGC |
| SD | GTTCTGGAGTTGTGCAGTTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 391 bp | - | + | + |
| TUF | TUR | 93 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 309 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10922C-H10 | VGB6 | C57BL/6NTac | |
| 10922A-F12 | VGB6 | C57BL/6NTac | |
