| Gene | Bex4 |
| Chromosome | X |
| Deletion Start | 136,139,863 |
| Deletion End | 136,140,622 |
| Deletion Size | 760 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 136,140,034 | 136,140,054 | AACTTTCTCTGGGCCATACCA |
| TUP | | | AGGCATGTTGATCGCA |
| TUR | 136,140,116 | 136,140,139 | CCGTAGTCTTTCTCTTGACTTCTG |
| TDF | 136,140,449 | 136,140,473 | GCTCAGTCATCTTCTACTTCAGTTC |
| TDP | 136,140,481 | 136,140,508 | TCCTTCATAGTGTCTTTCCTCTCCCAGC |
| TDR | 136,140,514 | 136,140,536 | GGATGTGAAGAAAAGCAGTGTCC |
PCR Primers
| Primer | Sequence |
| SU | TCTGAGAAGCAGAAGCTTCG |
| SD | TATGAGACTGGATAGGTTGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 106 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10925A-D11 | VGB6 | C57BL/6NTac | |
| 10925A-F2 | VGB6 | C57BL/6NTac | |
