| Gene | Ggt6 |
| Chromosome | 11 |
| Deletion Start | 72,435,603 |
| Deletion End | 72,438,184 |
| Deletion Size | 2,582 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,436,070 | 72,436,088 | GGTTGGGACTGGGCTACAC |
| TUP | 72,436,090 | 72,436,113 | TGGATGAACCCTGCTCCTCAACCA |
| TUR | 72,436,173 | 72,436,195 | GTCTAACAGAAGAGCTGGAGGAG |
| TDF | 72,437,890 | 72,437,908 | CTGGGCCTGTCCACTCATC |
| TDP | 72,437,911 | 72,437,937 | TCGTGACAACCTGGATGACACAGAAGC |
| TDR | 72,437,949 | 72,437,966 | TCCCTGAAGCCACCATCC |
PCR Primers
| Primer | Sequence |
| SU | CTGAGTAGCTTTGGGATCTC |
| SD | CACCCTGCCCAAAGACTCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 439 bp | - | + | + |
| TUF | TUR | 126 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 380 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10929A-A12 | VGB6 | C57BL/6NTac | |
| 10929A-B3 | VGB6 | C57BL/6NTac | |
| 10929A-B4 | VGB6 | C57BL/6NTac | |
| 10929A-E10 | VGB6 | C57BL/6NTac | |
