| Gene | Kcnk9 |
| Chromosome | 15 |
| Deletion Start | 72,546,276 |
| Deletion End | 72,546,007 |
| Deletion Size | 270 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 15
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 72,546,247 | 72,546,266 | ACGTGCGTACCCTGTCCTTG |
| TUP | 72,546,222 | 72,546,245 | TCGCCTGTACCTTCACCTACCTGC |
| TUR | 72,546,173 | 72,546,192 | CGCATCTCATGGTCCGACTC |
| TDF | 72,546,116 | 72,546,139 | TCTCAGAGGCAAGTACAACATCAG |
| TDP | 72,546,092 | 72,546,114 | TCCGATGACTACCAGCAGCTGGA |
| TDR | 72,546,030 | 72,546,049 | AGGAACCGGCGAATTTCCAC |
PCR Primers
| Primer | Sequence |
| SU | GAGACCCCTCAGCCTTCAGG |
| SD | CACTCTTCGCAAAAGTGGCG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 345 bp | - | + | + |
| TUF | TUR | 94 bp | + | + | - |
| TDF | TDR | 110 bp | + | + | - |
| NeoFwd | SD | 385 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10931A-E7 | VGB6 | C57BL/6NTac | |
| 10931A-H10 | VGB6 | C57BL/6NTac | |
