| Gene | B3galt4 |
| Chromosome | 17 |
| Deletion Start | 33,951,259 |
| Deletion End | 33,950,121 |
| Deletion Size | 1,139 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 17
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 33,950,820 | 33,950,839 | CGCAACCTCACCCTCAAGAC |
| TUP | 33,950,794 | 33,950,819 | CCTCAGTGGACTGAACTGGGTGAACA |
| TUR | 33,950,771 | 33,950,789 | GTAGCGGGCCATAGGACAG |
| TDF | 33,950,652 | 33,950,671 | GAGGCCCAGGAAGAGACAAC |
| TDP | 33,950,625 | 33,950,651 | AGCTATCCATGAAGAGCACAGAGGCCA |
| TDR | 33,950,592 | 33,950,614 | TGCACCCTGCCTAAATACAGAAG |
PCR Primers
| Primer | Sequence |
| SU | AGAGAGGCATGATGGGAAGG |
| SD | CATGCAGCAGTACTGAAAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 377 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 380 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10939A-F12 | VGB6 | C57BL/6NTac | |
| 10939A-E12 | VGB6 | C57BL/6NTac | |
