| Gene | Snx21 |
| Chromosome | 2 |
| Deletion Start | 164,786,052 |
| Deletion End | 164,792,418 |
| Deletion Size | 6,367 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,786,815 | 164,786,835 | CCAGCGCCAATGTTGTCAAAG |
| TUP | 164,786,836 | 164,786,859 | ACCCTCCCTCCAAGTACGTGGTGA |
| TUR | 164,786,872 | 164,786,891 | GACTCGCTGCTCAAGGGTTC |
| TDF | 164,792,222 | 164,792,241 | TCCTTTCCTGGCACCCTTTC |
| TDP | 164,792,242 | 164,792,263 | TTGAGGCCCATGTCCGGCTCTC |
| TDR | 164,792,280 | 164,792,298 | CCTCCGACTGCCGTTTGTC |
PCR Primers
| Primer | Sequence |
| SU | AGGCTGCTGCACCAAATCC |
| SD | TCCTCCTGGTGTTATCCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 362 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 246 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10940A-A3 | VGB6 | C57BL/6NTac | |
| 10940A-F4 | VGB6 | C57BL/6NTac | |
| 10940A-B4 | VGB6 | C57BL/6NTac | |
