| Gene | Zfp36l3 |
| Chromosome | X |
| Deletion Start | 53,776,242 |
| Deletion End | 53,774,042 |
| Deletion Size | 2,201 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome X
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 53,775,852 | 53,775,872 | CTGAGCTATGTCGGCCCTTTG |
| TUP | 53,775,825 | 53,775,851 | AGGAAAGCGGCATTTGCAAGTATGGCC |
| TUR | 53,775,806 | 53,775,823 | TGCGCGAACTGGCACTTG |
| TDF | 53,774,435 | 53,774,454 | ACTTCCAGGGCTGCCATCAC |
| TDP | 53,774,411 | 53,774,434 | TCCGGGAGTTGCTATTGCTCCAGG |
| TDR | 53,774,378 | 53,774,398 | GGAGCCAATATGCCGGTAGAG |
PCR Primers
| Primer | Sequence |
| SU | CTTTTAGCTCCTCCCAGCTC |
| SD | GAACCACACATTGCTTAACAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 306 bp | - | + | + |
| TUF | TUR | 67 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 366 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10945B-A1 | VGB6 | C57BL/6NTac | |
| 10945B-B3 | VGB6 | C57BL/6NTac | |
| 10945B-F5 | VGB6 | C57BL/6NTac | |
| 10945B-G12 | VGB6 | C57BL/6NTac | |
| 10945B-H1 | VGB6 | C57BL/6NTac | |
