| Gene | Ifi27l2a |
| Chromosome | 12 |
| Deletion Start | 103,443,630 |
| Deletion End | 103,442,307 |
| Deletion Size | 1,324 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 103,443,186 | 103,443,205 | GGCCCAGTGAAGGATGATTC |
| TUP | 103,443,162 | 103,443,185 | TTCTCAAGCCTGGGAACACCACGG |
| TUR | 103,443,118 | 103,443,137 | GGAGCAAGGTGGAGGCAAAG |
| TDF | 103,442,456 | 103,442,475 | GGCTGAAGCAAAGCCCTAAG |
| TDP | 103,442,428 | 103,442,453 | TCCCATAGAAATTGGGTGGGCATCTC |
| TDR | 103,442,404 | 103,442,423 | TGACAGGCAGTGACCTGATG |
PCR Primers
| Primer | Sequence |
| SU | GACAGAGTTCAGGAAAGATG |
| SD | GATTATTTCCATTAAGGCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 348 bp | - | + | + |
| TUF | TUR | 88 bp | + | + | - |
| TDF | TDR | 72 bp | + | + | - |
| NeoFwd | SD | 408 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10949A-A10 | VGB6 | C57BL/6NTac | |
| 10949A-F1 | VGB6 | C57BL/6NTac | |
| 10949A-F10 | VGB6 | C57BL/6NTac | |
| 10949A-H8 | VGB6 | C57BL/6NTac | |
