| Gene | Klhl9 |
| Chromosome | 4 |
| Deletion Start | 88,721,999 |
| Deletion End | 88,720,129 |
| Deletion Size | 1,871 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 88,721,400 | 88,721,419 | TTGAACGGCTTGCCTTTGTG |
| TUP | 88,721,369 | 88,721,396 | TCCAGCAATAGTCTCAAGCACTGTTCGG |
| TUR | 88,721,342 | 88,721,360 | GGCAGGCTGCCTTGAAGAG |
| TDF | 88,720,431 | 88,720,453 | ACTATTCGCCAACTCTTGACCAG |
| TDP | 88,720,406 | 88,720,430 | TGGACACCAATTGCTGCTATGCTGA |
| TDR | 88,720,377 | 88,720,398 | GACTGCAACTCCAACATCACTC |
PCR Primers
| Primer | Sequence |
| SU | GCTCCAAGCCAAGTCCGTAG |
| SD | ATCCTGACCTGTTTCTTCTAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 78 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10950A-B1 | VGB6 | C57BL/6NTac | |
| 10950B-C11 | VGB6 | C57BL/6NTac | |
| 10950D-G1 | VGB6 | C57BL/6NTac | |
