| Gene | Tcf23 |
| Chromosome | 5 |
| Deletion Start | 30,968,782 |
| Deletion End | 30,973,649 |
| Deletion Size | 4,868 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 5
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 30,968,861 | 30,968,882 | GCACTGACAGGAAGAGGAGTCG |
| TUP | 30,968,886 | 30,968,910 | CAACAGGACAAGGCAGGACCTGTGG |
| TUR | 30,968,969 | 30,968,988 | AGCTGTTCCCCTAGCTCTGG |
| TDF | 30,973,520 | 30,973,540 | GGGATGCTCTGACCTCGATTC |
| TDP | 30,973,542 | 30,973,564 | ACCACAGCTATCACGACCGGCCA |
| TDR | 30,973,591 | 30,973,610 | TGCGGCTACAGAGTCTTGAG |
PCR Primers
| Primer | Sequence |
| SU | CTTGGCCACTCCCAGCATGC |
| SD | TTGACTGAACCATGTCAACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 393 bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 91 bp | + | + | - |
| NeoFwd | SD | 377 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10953B-C5 | VGB6 | C57BL/6NTac | |
| 10953B-B7 | VGB6 | C57BL/6NTac | |
| 10953B-H12 | VGB6 | C57BL/6NTac | |
| 10953B-B10 | VGB6 | C57BL/6NTac | |
| 10953B-H8 | VGB6 | C57BL/6NTac | |
