| Gene | Pnma1 |
| Chromosome | 12 |
| Deletion Start | 84,147,924 |
| Deletion End | 84,146,814 |
| Deletion Size | 1,111 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,147,550 | 84,147,568 | TCGTGTGCTTGGGTTTCAG |
| TUP | 84,147,528 | 84,147,549 | AACCCTGCTCCAGCTCCAGGCC |
| TUR | 84,147,505 | 84,147,525 | GAGCATCTCTGCTGGCATTTC |
| TDF | 84,147,265 | 84,147,285 | CGCATCCTCAAGACCAACAAC |
| TDP | 84,147,240 | 84,147,262 | CGCCATAACGACCGCAGAATGCC |
| TDR | 84,147,209 | 84,147,231 | ACACTCCCAAAGACCTGCTCAAG |
PCR Primers
| Primer | Sequence |
| SU | ACAGCTGCAGCCAGTTCAG |
| SD | GCACAGCTGTCAGAGATGTC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 388 bp | - | + | + |
| TUF | TUR | 64 bp | + | + | - |
| TDF | TDR | 77 bp | + | + | - |
| NeoFwd | SD | 303 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10955A-C9 | VGB6 | C57BL/6NTac | |
| 10955A-D8 | VGB6 | C57BL/6NTac | |
| 10955A-F4 | VGB6 | C57BL/6NTac | |
