| Gene | Tctex1d1 |
| Chromosome | 4 |
| Deletion Start | 102,988,556 |
| Deletion End | 103,004,345 |
| Deletion Size | 15,790 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 4
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,988,895 | 102,988,912 | ATGGAGCACGCACTGGTC |
| TUP | 102,988,913 | 102,988,938 | TGCCAGAAGAGCCAAACTCAGTTCCC |
| TUR | 102,988,947 | 102,988,964 | GGTGGCTGTGAGCTGATG |
| TDF | 103,004,147 | 103,004,170 | AAGACCCAGGTCAAGGAATTAGTG |
| TDP | 103,004,189 | 103,004,217 | ATTGTCATCGTTTACATCGGACAGCGGGA |
| TDR | 103,004,239 | 103,004,259 | GGATTCCAGAGGCATCTGCTG |
PCR Primers
| Primer | Sequence |
| SU | GCTCTCCATGTCCAGTCTC |
| SD | AGGAAATTCTAGCTCTCCAAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 321 bp | - | + | + |
| TUF | TUR | 70 bp | + | + | - |
| TDF | TDR | 113 bp | + | + | - |
| NeoFwd | SD | 369 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10956A-G2 | VGB6 | C57BL/6NTac | |
| 10956A-H9 | VGB6 | C57BL/6NTac | |
