| Gene | Sgpp1 |
| Chromosome | 12 |
| Deletion Start | 75,735,560 |
| Deletion End | 75,715,937 |
| Deletion Size | 19,624 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 75,735,230 | 75,735,248 | GAGGGCGAGCTGGTCAAAG |
| TUP | 75,735,204 | 75,735,229 | TGAGCAACTTGCCGCTCTACTACCTG |
| TUR | 75,735,180 | 75,735,198 | GCCCAGTTCCGTGCCTAAG |
| TDF | 75,716,507 | 75,716,531 | GCATCTTCTCTTTCACCCTTGACAC |
| TDP | 75,716,483 | 75,716,505 | TGGAGCACATCCCGAGGAGACAC |
| TDR | 75,716,449 | 75,716,468 | TGCAATCCCAGCACCACTTC |
PCR Primers
| Primer | Sequence |
| SU | CAATTGGCACGTGGCTTTCC |
| SD | AACTAGGAAATGGCTCCCAG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 574 bp | - | + | + |
| TUF | TUR | 69 bp | + | + | - |
| TDF | TDR | 83 bp | + | + | - |
| NeoFwd | SD | 385 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10963C-C9 | VGB6 | C57BL/6NTac | |
| 10963C-E3 | VGB6 | C57BL/6NTac | |
| 10963C-F1 | VGB6 | C57BL/6NTac | |
| 10963C-G12 | VGB6 | C57BL/6NTac | |
