| Gene | Mrm1 |
| Chromosome | 11 |
| Deletion Start | 84,819,369 |
| Deletion End | 84,814,352 |
| Deletion Size | 5,018 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 11
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 84,818,875 | 84,818,892 | ATGCGCTCCGCTCACTTC |
| TUP | 84,818,850 | 84,818,873 | TCGGCGTAGACAGAGTCATCACCA |
| TUR | 84,818,828 | 84,818,845 | CGTCCGTGCCTGTTTCTC |
| TDF | 84,815,182 | 84,815,201 | TGCCCAGAGCAAATCCAGAC |
| TDP | 84,815,157 | 84,815,181 | CAGGTAGAGGTCCTGGCTGACTTCC |
| TDR | 84,815,117 | 84,815,136 | CCTTGCACACGCACATTCAC |
PCR Primers
| Primer | Sequence |
| SU | GGGAGCAGTCCAAGGTTAGC |
| SD | CCATCATCCCAAGAGACAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 65 bp | + | + | - |
| TDF | TDR | 85 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10967A-B2 | VGB6 | C57BL/6NTac | |
| 10967A-D1 | VGB6 | C57BL/6NTac | |
| 10967A-B9 | VGB6 | C57BL/6NTac | |
