10968   Tceal6     Status: ES cell colonies screened / QC positives

GeneTceal6
ChromosomeX
Deletion Start135,209,541
Deletion End135,208,538
Deletion Size1,004
Genome Build38
MGI IDMGI:1923939
NCBI Gene ID66104
BAC ID134D10
BAC LibrarybMQ
CassetteZEN-Ub1
Clones AvailableYes

Primer Genomic Coordinates on Chromosome X

Please be aware that these oligonucleotides have been validated only by Taqman assay.

PrimerStartEndSequence
TUF135,209,128135,209,149TGTAAGCAGCGAGGAGATGATG
TUP135,209,099135,209,127AGAGAGTGCACAGATATGACTAGGGCTCG
TUR135,209,076135,209,098TCTGCCTCTTCCTTAACTCTTCC
TDF135,208,731135,208,756CACCCATGTGCATGAAAAATATGTTC
TDP135,208,705135,208,729TGGTACATTGTAAAAATGAATAAAC
TDR135,208,668135,208,691GCAAACTGACACTTTATCTGAGGT

PCR Primers

PrimerSequence
SUCTGACCACACAGACACTGCC
SDGTCACAGTTTAGGTTTCAACG
LacInRTTGACTGTAGCGGCTGATGTTG
LacInfGGTAAACTGGCTCGGATTAGGG
NeoInRTACTTTCTCGGCAGGAGCAAGGTG
NeoInFTTCGGCTATGACTGGGCACAACAG
LacInZRevGTCTGTCCTAGCTTCCTCACTG
NeoFwdTCATTCTCAGTATTGTTTTGCC

Predicted PCR Products

Forward PrimerReverse PrimerLengthWTHETKO
SULacZRev bp-++
TUFTUR74 bp++-
TDFTDR89 bp++-
NeoFwdSD bp-++
LacInfLacInR210 bp-++
NeoInfNeoInR282 bp-++

Available Clones

ES Cell ordering information can be found at the KOMP Repository.
Clone NameParental ES CellMouse StrainCassette Copy Number
10968C-B9VGB6C57BL/6NTac
10968C-C5VGB6C57BL/6NTac
10968C-F2VGB6C57BL/6NTac
VELOCIGENE KOMP DESIGN DIAGRAMS
VelociGene KOMP Design
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VelociGene Genotyping Strategy

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