| Gene | Gcnt7 |
| Chromosome | 2 |
| Deletion Start | 172,454,899 |
| Deletion End | 172,451,001 |
| Deletion Size | 3,899 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 172,454,828 | 172,454,851 | TGCGCCTTCATCTTTCTTTACTTG |
| TUP | 172,454,802 | 172,454,825 | AAACCCAGGTCCTGAGGAGGCTGA |
| TUR | 172,454,749 | 172,454,769 | AGAAGCCACATTCCACCACAG |
| TDF | 172,451,139 | 172,451,159 | CACCGACTCAGAGCCCTACAG |
| TDP | 172,451,119 | 172,451,138 | CAGGCCGAGGTCCCTCCAGA |
| TDR | 172,451,059 | 172,451,080 | GTGCCTTAATGGTGAGGTTGAC |
PCR Primers
| Primer | Sequence |
| SU | AGTGAATGTGCTTCAGCAGG |
| SD | GAAGCTTGCTTCCTCTGTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 103 bp | + | + | - |
| TDF | TDR | 101 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10971A-A11 | VGB6 | C57BL/6NTac | |
| 10971A-D10 | VGB6 | C57BL/6NTac | |
