| Gene | Prr7 |
| Chromosome | 13 |
| Deletion Start | 55,472,011 |
| Deletion End | 55,473,021 |
| Deletion Size | 1,011 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 13
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 55,472,041 | 55,472,062 | GCACCTACACGTTCCTCACTTG |
| TUP | 55,472,063 | 55,472,086 | CTTCGCCGGCTTCTGGCTCATCTG |
| TUR | 55,472,089 | 55,472,108 | GCAGAGCAGGACGATGAGAC |
| TDF | 55,472,555 | 55,472,573 | GCGGCTTCATCCTCACCAG |
| TDP | 55,472,578 | 55,472,600 | CTTCCCGTTCCGCAGCGGAATCG |
| TDR | 55,472,606 | 55,472,623 | TAGCACGGCGGCTTAGAC |
PCR Primers
| Primer | Sequence |
| SU | TCGCTGAGCCCTGTGTCTG |
| SD | TGGTTTCCGAACACCAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 382 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 69 bp | + | + | - |
| NeoFwd | SD | 334 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10972A-A2 | VGB6 | C57BL/6NTac | |
| 10972A-A6 | VGB6 | C57BL/6NTac | |
| 10972A-A8 | VGB6 | C57BL/6NTac | |
| 10972A-F9 | VGB6 | C57BL/6NTac | |
| 10972A-D4 | VGB6 | C57BL/6NTac | |
| 10972A-E12 | VGB6 | C57BL/6NTac | |
