Gene | Doxl2 |
Chromosome | 6 |
Deletion Start | 48,981,082 |
Deletion End | 48,978,631 |
Deletion Size | 2,452 |
Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
Primer | Start | End | Sequence |
TUF | 48,975,381 | 48,975,400 | TGCTGCCCAACAAGAAGGTG |
TUP | 48,975,414 | 48,975,433 | TGAATAAAGGAACCAGACCT |
TUR | 48,975,488 | 48,975,508 | CCCTACAGCAAACTCGGTGAC |
TDF | 48,977,714 | 48,977,734 | CACATCTGAGCAGAGGCTGAC |
TDP | 48,977,735 | 48,977,758 | CCCTGAAACCCAAGAGGACTTGGC |
TDR | 48,977,779 | 48,977,799 | AGGTGCTCCCATGACTCACTC |
PCR Primers
Primer | Sequence |
SU | GTTCCTCAGTGTTATAACAC |
SD | GATATTGAAAAATCCATCAC |
LacInR | TTGACTGTAGCGGCTGATGTTG |
LacInf | GGTAAACTGGCTCGGATTAGGG |
NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
NeoInF | TTCGGCTATGACTGGGCACAACAG |
LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
Forward Primer | Reverse Primer | Length | WT | HET | KO |
SU | LacZRev | 6352 bp | - | + | + |
TUF | TUR | 128 bp | + | + | - |
TDF | TDR | 86 bp | + | + | - |
NeoFwd | SD | 345 bp | - | + | + |
LacInf | LacInR | 210 bp | - | + | + |
NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
10973B-A6 | VGB6 | C57BL/6NTac | |
10973B-B8 | VGB6 | C57BL/6NTac | |
10973B-C4 | VGB6 | C57BL/6NTac | |
10973B-F10 | VGB6 | C57BL/6NTac | |
10973B-H10 | VGB6 | C57BL/6NTac | |
10973B-C10 | VGB6 | C57BL/6NTac | |