| Gene | Doxl2 |
| Chromosome | 6 |
| Deletion Start | 48,981,082 |
| Deletion End | 48,978,631 |
| Deletion Size | 2,452 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 48,975,381 | 48,975,400 | TGCTGCCCAACAAGAAGGTG |
| TUP | 48,975,414 | 48,975,433 | TGAATAAAGGAACCAGACCT |
| TUR | 48,975,488 | 48,975,508 | CCCTACAGCAAACTCGGTGAC |
| TDF | 48,977,714 | 48,977,734 | CACATCTGAGCAGAGGCTGAC |
| TDP | 48,977,735 | 48,977,758 | CCCTGAAACCCAAGAGGACTTGGC |
| TDR | 48,977,779 | 48,977,799 | AGGTGCTCCCATGACTCACTC |
PCR Primers
| Primer | Sequence |
| SU | GTTCCTCAGTGTTATAACAC |
| SD | GATATTGAAAAATCCATCAC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 6352 bp | - | + | + |
| TUF | TUR | 128 bp | + | + | - |
| TDF | TDR | 86 bp | + | + | - |
| NeoFwd | SD | 345 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10973B-A6 | VGB6 | C57BL/6NTac | |
| 10973B-B8 | VGB6 | C57BL/6NTac | |
| 10973B-C4 | VGB6 | C57BL/6NTac | |
| 10973B-F10 | VGB6 | C57BL/6NTac | |
| 10973B-H10 | VGB6 | C57BL/6NTac | |
| 10973B-C10 | VGB6 | C57BL/6NTac | |
