| Gene | Rin3 |
| Chromosome | 12 |
| Deletion Start | 102,283,289 |
| Deletion End | 102,313,178 |
| Deletion Size | 29,890 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 102,284,132 | 102,284,152 | CACTGCCCAGAGCTACACAAG |
| TUP | 102,284,159 | 102,284,182 | AATGTCCTGTCATCCCGCCCAGCC |
| TUR | 102,284,188 | 102,284,210 | CACCCTTCCCGATCTTCTCTTAG |
| TDF | 102,312,392 | 102,312,412 | CCGCTGTTTCCACTCTACTTC |
| TDP | 102,312,419 | 102,312,448 | ATTTAGTCCCTTCTTGTACCGTAGCTGGCC |
| TDR | 102,312,477 | 102,312,498 | GTCTCCACGTAAGGCATTTGTC |
PCR Primers
| Primer | Sequence |
| SU | GATGAAGGAGGTCCCATTTG |
| SD | TCTGCTGATGAACCACATGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 107 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10977A-F9 | VGB6 | C57BL/6NTac | |
| 10977A-E9 | VGB6 | C57BL/6NTac | |
| 10977A-A1 | VGB6 | C57BL/6NTac | |
| 10977A-B10 | VGB6 | C57BL/6NTac | |
