| Gene | Wfdc12 |
| Chromosome | 2 |
| Deletion Start | 164,190,516 |
| Deletion End | 164,189,659 |
| Deletion Size | 858 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 2
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 164,190,176 | 164,190,195 | ATGCTGCTTCTGGCAATGTG |
| TUP | 164,190,152 | 164,190,174 | CTTCAAATGCGTGCTGCCGGTGA |
| TUR | 164,190,119 | 164,190,142 | CACTGGGAGCTACTTACTCTTCAC |
| TDF | 164,189,980 | 164,189,999 | GGCCTTGACCGTCTCCTTTG |
| TDP | 164,189,945 | 164,189,969 | AAGGCGTGACAGGGATGTCAACCAA |
| TDR | 164,189,925 | 164,189,944 | GGGCTTTCACCTGATTCTGC |
PCR Primers
| Primer | Sequence |
| SU | GAAGAAGTAAGACCTCTGTC |
| SD | ACACATAATATTGAACAAGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 547 bp | - | + | + |
| TUF | TUR | 77 bp | + | + | - |
| TDF | TDR | 75 bp | + | + | - |
| NeoFwd | SD | 355 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10984B-D6 | VGB6 | C57BL/6NTac | |
| 10984B-E12 | VGB6 | C57BL/6NTac | |
