| Gene | Lmod2 |
| Chromosome | 6 |
| Deletion Start | 24,597,867 |
| Deletion End | 24,605,212 |
| Deletion Size | 7,346 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 6
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 24,597,980 | 24,597,999 | TTGAGAGGGAGCTGGAAGAC |
| TUP | 24,598,002 | 24,598,027 | TGAACCTGACCGAAACCTTCCTGTGG |
| TUR | 24,598,061 | 24,598,081 | CTCGGCTGAAGTTTCCTGTTG |
| TDF | 24,604,225 | 24,604,244 | CCGAGACAGTCTCCCTGGTC |
| TDP | 24,604,249 | 24,604,277 | CCCAAAGTCTCCAAGAAAGTCCACACTGG |
| TDR | 24,604,286 | 24,604,304 | GCCACAGGAGATGGAGGTC |
PCR Primers
| Primer | Sequence |
| SU | ACCTCCATCCACCACTGATG |
| SD | ATAGCATATTTCTTTAAGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 376 bp | - | + | + |
| TUF | TUR | 102 bp | + | + | - |
| TDF | TDR | 80 bp | + | + | - |
| NeoFwd | SD | 375 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10985A-B9 | VGB6 | C57BL/6NTac | |
| 10985A-F1 | VGB6 | C57BL/6NTac | |
| 10985A-C6 | VGB6 | C57BL/6NTac | |
| 10985B-B4 | VGB6 | C57BL/6NTac | |
| 10985B-C3 | VGB6 | C57BL/6NTac | |
| 10985B-C11 | VGB6 | C57BL/6NTac | |
| 10985B-G6 | VGB6 | C57BL/6NTac | |
