| Gene | Nudt14 |
| Chromosome | 12 |
| Deletion Start | 112,942,068 |
| Deletion End | 112,934,723 |
| Deletion Size | 7,346 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 112,941,811 | 112,941,831 | GCCAAGGAAGGACATGCAAAG |
| TUP | 112,941,777 | 112,941,805 | CGCTCTTTCCTGAGGAAGATTTGACTGGC |
| TUR | 112,941,753 | 112,941,772 | TCGATGGGCGGTCAGAAATG |
| TDF | 112,934,936 | 112,934,957 | GGAGGGAGAACTCATCGAAGTG |
| TDP | 112,934,906 | 112,934,932 | CATCTGAATTTAGATGACGCCCAGGCC |
| TDR | 112,934,828 | 112,934,849 | GGGAACCACCTGGCTAAAGAAC |
PCR Primers
| Primer | Sequence |
| SU | GCTCCCCAGACTTGGGAAGG |
| SD | AGCTGATATGGAACTTGATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 462 bp | - | + | + |
| TUF | TUR | 79 bp | + | + | - |
| TDF | TDR | 130 bp | + | + | - |
| NeoFwd | SD | 336 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10992A-C8 | VGB6 | C57BL/6NTac | |
| 10992A-D1 | VGB6 | C57BL/6NTac | |
| 10992A-E8 | VGB6 | C57BL/6NTac | |
| 10992A-H1 | VGB6 | C57BL/6NTac | |
