| Gene | Fabp9 |
| Chromosome | 3 |
| Deletion Start | 10,197,161 |
| Deletion End | 10,193,734 |
| Deletion Size | 3,428 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 3
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 10,196,674 | 10,196,693 | AGAAGCACAGTGGCAACAAC |
| TUP | 10,196,638 | 10,196,665 | CAGCTGTCCTCAACCTTCCTAATGCTGG |
| TUR | 10,196,603 | 10,196,623 | GGCCATGACAACATGAGAAAC |
| TDF | 10,193,898 | 10,193,921 | ACTACCCACCAAGCTACTCATTTG |
| TDP | 10,193,875 | 10,193,897 | TCTCTGCGGCCTGAATTGAGGCT |
| TDR | 10,193,825 | 10,193,844 | GGCGTGACTGATGACAGGTC |
PCR Primers
| Primer | Sequence |
| SU | CTAGAAGCTTAGAGAGGCTC |
| SD | AGTCGTGCAGGGCATAGCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 352 bp | - | + | + |
| TUF | TUR | 91 bp | + | + | - |
| TDF | TDR | 97 bp | + | + | - |
| NeoFwd | SD | 259 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 10999A-C11 | VGB6 | C57BL/6NTac | |
| 10999A-F7 | VGB6 | C57BL/6NTac | |
| 10999A-E7 | VGB6 | C57BL/6NTac | |
