| Gene | Pah |
| Chromosome | 10 |
| Deletion Start | 87,522,020 |
| Deletion End | 87,538,319 |
| Deletion Size | 16,300 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 87,522,835 | 87,522,855 | GCCCAAGGATTCTGGCTCTAC |
| TUP | 87,522,864 | 87,522,891 | AAGCTCCTATATTGCAGGCTGCACAGTC |
| TUR | 87,522,915 | 87,522,934 | CCCACTCCCAGATTTCTGTC |
| TDF | 87,538,240 | 87,538,258 | CGTCCTGGGCAGCATCATC |
| TDP | 87,538,262 | 87,538,286 | AGCCTGAGGAACGACATTGGTGCCA |
| TDR | 87,538,287 | 87,538,306 | TCGGGAAAGCTCATGGACAG |
PCR Primers
| Primer | Sequence |
| SU | TCAGTCCAGGACTGGAGTTG |
| SD | TCAGTGAGTGTAAGAAACAGC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 100 bp | + | + | - |
| TDF | TDR | 67 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11003A-B6 | VGB6 | C57BL/6NTac | |
| 11003A-B7 | VGB6 | C57BL/6NTac | |
