| Gene | Zfp474 |
| Chromosome | 18 |
| Deletion Start | 52,638,261 |
| Deletion End | 52,639,333 |
| Deletion Size | 1,073 |
| Genome Build | 38 |
|
|
Primer Genomic Coordinates on Chromosome 18
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 52,638,619 | 52,638,639 | GAGAAGTGGCGCACTGAGAAC |
| TUP | 52,638,642 | 52,638,667 | CAAACTACCCAAGCACCTGAGGAGGC |
| TUR | 52,638,675 | 52,638,692 | ATGGGCTGCGGTTTGGAG |
| TDF | 52,638,817 | 52,638,837 | CAGAGAAGCTGCAAGCCAAAG |
| TDP | 52,638,840 | 52,638,862 | TGAGAACCCTGGACCACCAAGCA |
| TDR | 52,638,869 | 52,638,892 | TGAGACCAGTAGGAACATTAGAAC |
PCR Primers
| Primer | Sequence |
| SU | CCTGTTCCTTACAGATCTTG |
| SD | GGGATCAGAGAAAACAGTCC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 335 bp | - | + | + |
| TUF | TUR | 74 bp | + | + | - |
| TDF | TDR | 76 bp | + | + | - |
| NeoFwd | SD | 338 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11006B-C8 | VGB6 | C57BL/6NTac | |
| 11006B-H5 | VGB6 | C57BL/6NTac | |
