| Gene | Ppp1r14c |
| Chromosome | 10 |
| Deletion Start | 3,366,669 |
| Deletion End | 3,366,964 |
| Deletion Size | 296 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 10
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 3,366,734 | 3,366,717 | GGTGCGCGTGTCTTCTTC |
| TUP | 3,366,755 | 3,366,735 | CAGAGTCCCCGCGGTGGAACC |
| TUR | 3,366,808 | 3,366,791 | GCCGAGTCCTCCCTTGAG |
| TDF | 3,366,866 | 3,366,847 | AGCAACAACGACGCCACCAG |
| TDP | 3,366,906 | 3,366,877 | TGACAGTGAAATACGACCGTAAGGAGCTTC |
| TDR | 3,366,945 | 3,366,927 | GCTGCTCCACGATCCATTC |
PCR Primers
| Primer | Sequence |
| SU | GTCTCCTCCTTGGACTACAC |
| SD | AGAACACGGAGGAAAGCATC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TUF | TUR | 92 bp | + | + | - |
| TDF | TDR | 99 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11007A-F7 | VGB6 | C57BL/6NTac | |
| 11007A-E5 | VGB6 | C57BL/6NTac | |
| 11007A-G2 | VGB6 | C57BL/6NTac | |
