| Gene | Ttc9 |
| Chromosome | 12 |
| Deletion Start | 81,631,391 |
| Deletion End | 81,663,599 |
| Deletion Size | 32,209 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 12
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 81,632,166 | 81,632,187 | GAAGCCAGAGATACCGCACAAG |
| TUP | 81,632,190 | 81,632,216 | ATGGGCTCTAAGGCTAGCTCATGCACC |
| TUR | 81,632,272 | 81,632,292 | GGCCTGTTTCCATCTGGTTAC |
| TDF | 81,663,211 | 81,663,230 | GGAGCTTGCCTGAGGGTTAG |
| TDP | 81,663,231 | 81,663,253 | TGCCAAGCCGACTCTCAGCTAAC |
| TDR | 81,663,275 | 81,663,298 | CCTGACACCGGAAGTAGTTATCTC |
PCR Primers
| Primer | Sequence |
| SU | GTTGCGGGAGCAGAAAAGG |
| SD | GCTGTCCCATTTGCTGACC |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 361 bp | - | + | + |
| TUF | TUR | 127 bp | + | + | - |
| TDF | TDR | 88 bp | + | + | - |
| NeoFwd | SD | 318 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11008A-C6 | VGB6 | C57BL/6NTac | |
| 11008A-C11 | VGB6 | C57BL/6NTac | |
| 11008A-G9 | VGB6 | C57BL/6NTac | |
| 11008A-H12 | VGB6 | C57BL/6NTac | |
