| Gene | Sprn |
| Chromosome | 7 |
| Deletion Start | 140,153,654 |
| Deletion End | 140,153,212 |
| Deletion Size | 443 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 7
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | | | GCCGCCTTCCTCTGTGA |
| TUP | 140,153,552 | 140,153,571 | AGGCGCTCGAGGCAGTGCCC |
| TUR | | | CGCAGAGAGGAGCCGTA |
| TDF | 140,153,354 | 140,153,374 | GCCTGGAGGACGATGAGAATG |
| TDP | 140,153,327 | 140,153,350 | CAATGGGAGGCAACGGAACCGACC |
| TDR | 140,153,302 | 140,153,321 | CAGGCCCAGTAGCTGTAGAC |
PCR Primers
| Primer | Sequence |
| SU | CAGAGACTCGTGTGCAGAGC |
| SD | GCATTGTTCATGTCCTCGTG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | bp | - | + | + |
| TDF | TDR | 73 bp | + | + | - |
| NeoFwd | SD | bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11009A-A6 | VGB6 | C57BL/6NTac | |
| 11009A-B1 | VGB6 | C57BL/6NTac | |
| 11009A-C4 | VGB6 | C57BL/6NTac | |
| 11009A-C12 | VGB6 | C57BL/6NTac | |
| 11009A-F5 | VGB6 | C57BL/6NTac | |
| 11009A-G11 | VGB6 | C57BL/6NTac | |
