| Gene | Teddm1 |
| Chromosome | 1 |
| Deletion Start | 153,891,776 |
| Deletion End | 153,892,709 |
| Deletion Size | 934 |
| Genome Build | 38 |
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Primer Genomic Coordinates on Chromosome 1
Please be aware that these oligonucleotides have been validated only by Taqman assay.
| Primer | Start | End | Sequence |
| TUF | 153,892,082 | 153,892,100 | GCAGCACCTCACCATGTTC |
| TUP | 153,892,102 | 153,892,127 | TCCTTCTCACCCTTGATGGCTGTGTA |
| TUR | 153,892,128 | 153,892,149 | GCATGTTCTTGCTCACCATTTC |
| TDF | 153,892,349 | 153,892,373 | GATAGAGACCTTCCTGATCCTGATA |
| TDP | 153,892,374 | 153,892,397 | ATGGGCTCGTGGCTGATTCAGGCA |
| TDR | 153,892,407 | 153,892,430 | GAAGCCAGAGACTGGTCTAAAGAG |
PCR Primers
| Primer | Sequence |
| SU | ATGAGCCAAAGCCACTCAGG |
| SD | CGCTGAGTGCAGAATTCTGG |
| LacInR | TTGACTGTAGCGGCTGATGTTG |
| LacInf | GGTAAACTGGCTCGGATTAGGG |
| NeoInR | TACTTTCTCGGCAGGAGCAAGGTG |
| NeoInF | TTCGGCTATGACTGGGCACAACAG |
| LacInZRev | GTCTGTCCTAGCTTCCTCACTG |
| NeoFwd | TCATTCTCAGTATTGTTTTGCC |
Predicted PCR Products
| Forward Primer | Reverse Primer | Length | WT | HET | KO |
| SU | LacZRev | 359 bp | - | + | + |
| TUF | TUR | 68 bp | + | + | - |
| TDF | TDR | 82 bp | + | + | - |
| NeoFwd | SD | 373 bp | - | + | + |
| LacInf | LacInR | 210 bp | - | + | + |
| NeoInf | NeoInR | 282 bp | - | + | + |
Available Clones
ES Cell ordering information can be found at the
KOMP Repository.
| Clone Name | Parental ES Cell | Mouse Strain | Cassette Copy Number |
| 11011A-A10 | VGB6 | C57BL/6NTac | |
| 11011A-C8 | VGB6 | C57BL/6NTac | |
| 11011A-D9 | VGB6 | C57BL/6NTac | |
| 11011A-H9 | VGB6 | C57BL/6NTac | |
